1G9Z
LAGLIDADG HOMING ENDONUCLEASE I-CREI / DNA PRODUCT COMPLEX WITH MAGNESIUM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-04-01 |
| Detector | ADSC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.000, 67.900, 88.300 |
| Unit cell angles | 90.00, 91.60, 90.00 |
Refinement procedure
| Resolution | 19.920 - 1.800 |
| Rwork | 0.204 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | CRE/DNA (PDB ENTRY 1BP7) |
| RMSD bond length | 0.005 |
| RMSD bond angle | 20.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.890 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.038 | 0.185 |
| Number of reflections | 44925 | |
| <I/σ(I)> | 21.3 | |
| Completeness [%] | 95.4 | 75.3 |
| Redundancy | 3.5 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 30% PEG400, PH 6.5, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG400 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 10 (mM) | ||
| 2 | 1 | drop | protein | 3.5 (mg/ml) | |
| 3 | 1 | reservoir | 20 (mM) | ||
| 4 | 1 | reservoir | MES | 100 (mM) | |
| 5 | 1 | reservoir | PEG400 | 20-35 (%(v/v)) |
