1G9Y
HOMING ENDONUCLEASE I-CREI / DNA SUBSTRATE COMPLEX WITH CALCIUM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-05-01 |
| Detector | ADSC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.200, 67.800, 88.300 |
| Unit cell angles | 90.00, 91.60, 90.00 |
Refinement procedure
| Resolution | 26.990 - 2.050 |
| Rwork | 0.207 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | CRE/DNA (PDB ENTRY 1BP7) |
| RMSD bond length | 0.005 |
| RMSD bond angle | 20.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.090 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.055 | 0.360 |
| Number of reflections | 30037 | |
| <I/σ(I)> | 13.1 | |
| Completeness [%] | 91.4 | 81.7 |
| Redundancy | 3.2 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 25% PEG 400, pH 6.5, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 400 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 10 (mM) | ||
| 2 | 1 | drop | protein | 3.5 (mg/ml) | |
| 3 | 1 | reservoir | 20 (mM) | ||
| 4 | 1 | reservoir | MES | 100 (mM) | |
| 5 | 1 | reservoir | PEG400 | 20-35 (%(v/v)) |
