1G9S
CRYSTAL STRUCTURE OF A COMPLEX BETWEEN E.COLI HPRT AND IMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-D |
Synchrotron site | APS |
Beamline | 14-BM-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-12-16 |
Detector | ADSC QUANTUM 1 |
Wavelength(s) | 1.0 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 84.140, 84.140, 167.270 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.800 |
R-factor | 0.201 |
Rwork | 0.201 |
R-free | 0.24300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | T.foetus HPRT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.370 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.071 | 0.276 |
Total number of observations | 58776 * | |
Number of reflections | 17473 * | |
<I/σ(I)> | 8.7 | 4.5 |
Completeness [%] | 99.7 | 99.8 |
Redundancy | 3.4 | 1.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 * | 20 * | Hepes, sodium citrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 18 (mg/ml) | 0.88mM in terms of subumits |
2 | 1 | drop | Tris-HCl | 0.05 (M) | |
3 | 1 | drop | 0.01 (M) | ||
4 | 1 | drop | dithiothreitol | 1 (mM) | pH7.4 |
5 | 1 | reservoir | HEPES | 0.1 (M) | pH7.5 |
6 | 1 | reservoir | sodium citrate | 0.1 (M) |