1G9R

CRYSTAL STRUCTURE OF GALACTOSYLTRANSFERASE LGTC IN COMPLEX WITH MN AND UDP-2F-GALACTOSE

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL1-5
Synchrotron site	SSRL
Beamline	BL1-5
Temperature [K]	100
Detector technology	CCD
Collection date	2000-05-05
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97996, 0.97949, 0.92526, 1.06883
Spacegroup name	P 21 21 21
Unit cell lengths	39.790, 76.050, 86.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 2.000
R-factor	0.199 *
Rwork	0.193
R-free	0.22740 *
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.310 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.044	0.113
Number of reflections	18244
<I/σ(I)>	19.8	6
Completeness [%]	99.0	97.7
Redundancy	12.0 *	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7 *	291	used seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	TCEP	3 (mM)
2	1	drop		3 (mM)
3	1	drop	UDP-2FGal	5 (mM)
4	1	drop	protein	10 (mg/ml)
5	1	drop		50 (mM)
6	1	reservoir		50 (mM)
7	1	reservoir	PEG2000 MME	5-20 (%(w/v))