1G9R
CRYSTAL STRUCTURE OF GALACTOSYLTRANSFERASE LGTC IN COMPLEX WITH MN AND UDP-2F-GALACTOSE
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL1-5 |
| Synchrotron site | SSRL |
| Beamline | BL1-5 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-05-05 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97996, 0.97949, 0.92526, 1.06883 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.790, 76.050, 86.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 2.000 |
| R-factor | 0.199 * |
| Rwork | 0.193 |
| R-free | 0.22740 * |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.310 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.044 | 0.113 |
| Number of reflections | 18244 | |
| <I/σ(I)> | 19.8 | 6 |
| Completeness [%] | 99.0 | 97.7 |
| Redundancy | 12.0 * | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 291 | used seeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | TCEP | 3 (mM) | |
| 2 | 1 | drop | 3 (mM) | ||
| 3 | 1 | drop | UDP-2FGal | 5 (mM) | |
| 4 | 1 | drop | protein | 10 (mg/ml) | |
| 5 | 1 | drop | 50 (mM) | ||
| 6 | 1 | reservoir | 50 (mM) | ||
| 7 | 1 | reservoir | PEG2000 MME | 5-20 (%(w/v)) |
