1G95
CRYSTAL STRUCTURE OF S.PNEUMONIAE GLMU, APO FORM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM1A |
Synchrotron site | ESRF |
Beamline | BM1A |
Temperature [K] | 120 |
Detector technology | IMAGE PLATE |
Collection date | 1998-04-26 |
Detector | MARRESEARCH |
Wavelength(s) | 0.873 |
Spacegroup name | H 3 2 |
Unit cell lengths | 94.910, 94.910, 279.940 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.330 |
R-factor | 0.23 * |
Rwork | 0.228 |
R-free | 0.30300 |
Structure solution method | SIRAS |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.410 |
High resolution limit [Å] | 2.330 | 2.330 |
Rmerge | 0.061 * | 0.188 * |
Number of reflections | 20586 | |
<I/σ(I)> | 10.5 | 3.1 |
Completeness [%] | 97.0 | 95.7 |
Redundancy | 1.8 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 6.5 | 298 | 0.1 M BIS/TRIS, 0.2 M ammonium sulfate, 25 % PEG 3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 14 (mg/ml) | |
2 | 1 | reservoir | Bis-Tris | 0.1 (M) | |
3 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
4 | 1 | reservoir | PEG3350 | 25 (%(w/v)) |