1G8X
STRUCTURE OF A GENETICALLY ENGINEERED MOLECULAR MOTOR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID13 |
Synchrotron site | ESRF |
Beamline | ID13 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-01-01 |
Detector | MARRESEARCH |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 135.423, 155.420, 143.191 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.150 - 2.800 |
R-factor | 0.232 |
Rwork | 0.232 |
R-free | 0.29000 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.300 |
Data scaling software | XDS |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 * | |
High resolution limit [Å] | 2.800 * | 2.800 * |
Rmerge | 0.110 * | 0.324 * |
Number of reflections | 73355 * | |
Completeness [%] | 97.7 * | 98 * |
Redundancy | 3.8 * | 3.9 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.3 * | 7 * | 12% PEGM 5000; 170 mM NaCl; 50 mM HEPES (NaOH) pH 7.2; 5 mM MgCl2; 5 mM DTT; 0.5 mM EGTA; and 2% 2-methyl-1,3-propanediol, at 280 K, VAPOR DIFFUSION, SITTING DROP |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
10 | 1 | reservoir | HEPES-NaOH | 50 (mM) | |
11 | 1 | reservoir | 5 (mM) | ||
12 | 1 | reservoir | dithiothreitol | 5 (mM) | |
13 | 1 | reservoir | EGTA | 0.5 (mM) | |
14 | 1 | reservoir | 2-methyl-1,3-propanediol | 2 (%) | |
2 | 1 | drop | HEPES-NaOH | 30 (mM) | |
3 | 1 | drop | EDTA | 0.5 (mM) | |
4 | 1 | drop | dithiothreitol | 2 (mM) | |
5 | 1 | drop | benzamidine | 1 (mM) | |
6 | 1 | drop | 1 (mM) | ||
7 | 1 | drop | sucrose | 3 (%) | |
8 | 1 | reservoir | mPEG5000 | 12 (%) | |
9 | 1 | reservoir | 170 (mM) |