1G8S
METHANOCOCCUS JANNASCHII FIBRILLARIN PRE-RRNA PROCESSING PROTEIN
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 170 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-10-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97894 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 121.152, 43.212, 55.197 |
| Unit cell angles | 90.00, 96.98, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.600 |
| R-factor | 0.2016 |
| Rwork | 0.201 |
| R-free | 0.21300 |
| Structure solution method | MAD |
| Starting model (for MR) | 1fbn |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO (SUITE) |
| Data scaling software | SCALEPACK (SUITE) |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.076 | 0.400 |
| Number of reflections | 68772 | |
| Completeness [%] | 97.0 | 95.8 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 273 | 20% PEG 4000, 20% Isopropanol, 0.1 M NaCitrate, 25 mg/ml fibrillarin, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 273K |
