1G8E
CRYSTAL STRUCTURE OF FLHD FROM ESCHERICHIA COLI
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-08-23 |
| Detector | APS-1 |
| Wavelength(s) | 0.9464,0.9793,0.9794,1.0332 |
| Spacegroup name | I 4 |
| Unit cell lengths | 88.717, 88.717, 42.423 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.800 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.29900 |
| Structure solution method | MAD |
| Starting model (for MR) | none |
| RMSD bond length | 0.019 |
| RMSD bond angle | 16.800 * |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 1.880 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.034 | 0.318 |
| Total number of observations | 50417 * | |
| Number of reflections | 15124 | |
| <I/σ(I)> | 21.4 | 2.5 |
| Completeness [%] | 97.4 | 79.6 |
| Redundancy | 3.33 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.9 * | 20 * | 0.1 M Tris, 0.2 M sodium acetate, 30% PEG 5000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 40 (mg/ml) | |
| 2 | 1 | drop | Tris | 50 (mM) | |
| 3 | 1 | reservoir | PEG5000 | 20-30 (%) | |
| 4 | 1 | reservoir | sodium acetate | 0.05-0.2 (M) | |
| 5 | 1 | reservoir | Tris-HCl | 0.1 (M) |
