1G7Y
THE CRYSTAL STRUCTURE OF THE 58KD VEGETATIVE LECTIN FROM THE TROPICAL LEGUME DOLICHOS BIFLORUS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-08-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.98089 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 100.941, 130.272, 137.124 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | non-canonical legume lectin dimer (non-canonical half of a DBL tetramer) |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.104 | 0.656 |
| Total number of observations | 276382 * | |
| Number of reflections | 59075 * | |
| <I/σ(I)> | 12.9 | 2.78 |
| Completeness [%] | 93.8 | 95.7 |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 5-7 mg/ml protein; 100mM sodium citrate; 8-12%(w/v) PEG(6000); 0.2-0.4M sodium chloride, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | protein | 5-7 (mg/ml) | |
| 2 | 1 | reservoir | sodium citrate | 100 (mM) | |
| 3 | 1 | reservoir | PEG6000 | 8-12 (%(w/v)) | |
| 4 | 1 | reservoir | 0.2-0.4 (M) |
