1G7W
ASPARTATE AMINOTRANSFERASE ACTIVE SITE MUTANT N194A/R386L
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-08-14 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 154.940, 89.330, 79.960 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.200 |
| R-factor | 0.217 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.830 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.200 |
| Rmerge | 0.067 * |
| Total number of observations | 127902 * |
| Number of reflections | 28430 |
| <I/σ(I)> | 21.9 |
| Completeness [%] | 93.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Sodium Sulfate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 40-60 (mg/ml) | |
| 2 | 1 | drop | potassium phosphate | 10 (mM) | |
| 3 | 1 | drop | PLP | 0.010 (mM) | |
| 4 | 1 | drop | 0.3 (mM) | ||
| 5 | 1 | reservoir | potassium phosphate | 10 (mM) | |
| 6 | 1 | reservoir | PLP | 0.010 (mM) | |
| 7 | 1 | reservoir | 0.3 (mM) | ||
| 8 | 1 | reservoir | ammonium sulfate | 40 (%sat) |
