1G7T

X-RAY STRUCTURE OF TRANSLATION INITIATION FACTOR IF2/EIF5B COMPLEXED WITH GDPNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	95
Detector technology	CCD
Collection date	2000-04-28
Detector	BRANDEIS
Wavelength(s)	0.9500
Spacegroup name	P 1
Unit cell lengths	48.799, 53.680, 94.926
Unit cell angles	105.17, 94.76, 100.17

Refinement procedure

Resolution	22.000 - 2.000
Rwork	0.231
R-free	0.26700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.000	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.066	0.170
Number of reflections	58931
<I/σ(I)>	17
Completeness [%]	97.2	86
Redundancy	6	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8 *	293	0.1M Cacodylate pH 5.6, 18% PEG4000, 0.2M Lithium Sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	cacodylate	100 (mM)
3	1	reservoir	PEG4000	18 (%)
4	1	reservoir		0.2 (M)