1G7R

X-RAY STRUCTURE OF TRANSLATION INITIATION FACTOR IF2/EIF5B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9B
Synchrotron site	NSLS
Beamline	X9B
Temperature [K]	95
Detector technology	CCD
Collection date	2000-03-15
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9686
Spacegroup name	P 1
Unit cell lengths	48.316, 54.355, 90.978
Unit cell angles	104.90, 101.33, 98.75

Refinement procedure

Resolution	22.000 - 2.200
Rwork	0.243
R-free	0.27500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.700
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.000	2.250
High resolution limit [Å]	2.200	2.200
Rmerge	0.023	0.150
Number of reflections	79984
<I/σ(I)>	30
Completeness [%]	92.0	92
Redundancy	4	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	298	0.1M Cacodylate 5.8, 18% PEG4000, 0.2M Lithium Sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop		0.2 (M)
3	1	reservoir	cacodylate	100 (mM)
4	1	reservoir	PEG4000	18 (%)