1G7N

Toward changing specificity: adipocyte lipid binding protein mutant, apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1998-03-02
Detector	SIEMENS
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	120.080, 37.970, 28.820
Unit cell angles	90.00, 92.32, 90.00

Refinement procedure

Resolution	8.000 - 1.500
Rwork	0.196
R-free	0.22500 *
Starting model (for MR)	PDB 1LIB without waters ligand alternative conformations or ions
RMSD bond length	0.006
RMSD bond angle	26.800 *
Data reduction software	X-GEN
Data scaling software	X-GEN
Phasing software	X-PLOR
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	8.000 *	1.590
High resolution limit [Å]	1.500	1.500
Rmerge	0.045 *	0.229
Total number of observations	83041 *
Number of reflections	31800 *
<I/σ(I)>	34.5
Completeness [%]	89.0 *	37.6
Redundancy	2.61 *	0.54

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2 *	291	sodium/potassium phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	9.0 (mg/ml)
2	1	drop	HEPES	12.5 (M)	pH7.2
3	1	reservoir	sodium potassium phosphate	1.5-3 (M)
4	1	reservoir	PEG4000	24-34 (%)
5	1	reservoir		0.1-0.2 (M)	pH7.4
6	1	reservoir	ammonium sulfate	0.1 (M)