1G7M

CRYSTAL STRUCTURE OF HEN EGG WHITE LYSOZYME (HEL) COMPLEXED WITH THE MUTANT ANTI-HEL MONOCLONAL ANTIBODY D1.3 (VLW92V)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	SIEMENS
Temperature [K]	298
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	123.670, 60.400, 56.790
Unit cell angles	90.00, 114.25, 90.00

Refinement procedure

Resolution	15.000 - 1.900
R-factor	0.1941 *
Rwork	0.194
R-free	0.22560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.267
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	1.940
High resolution limit [Å]	1.900	1.900
Rmerge	0.054	0.308
Total number of observations	84329 *
Number of reflections	27663
Completeness [%]	91.7	87

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	used macroseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	14 (%(w/v))
2	1	reservoir	sodium acetate	0.1 (M)
3	1	reservoir	sodium cacodylate	0.05 (M)