1G7L

CRYSTAL STRUCTURE OF HEN EGG WHITE LYSOZYME (HEL) COMPLEXED WITH THE MUTANT ANTI-HEL MONOCLONAL ANTIBODY D1.3 (VLW92S)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	SIEMENS
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	121.630, 59.060, 55.860
Unit cell angles	90.00, 113.59, 90.00

Refinement procedure

Resolution	15.000 - 2.000
R-factor	0.207 *
Rwork	0.207
R-free	0.24030
RMSD bond length	0.008
RMSD bond angle	1.528
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.051	0.350
Total number of observations	53007 *
Number of reflections	23556
Completeness [%]	95.7	93.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	used macroseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	15-18 (%(w/v))
2	1	reservoir	potassium phosphate	0.1 (M)