1G7K

CRYSTAL STRUCTURE OF DSRED, A RED FLUORESCENT PROTEIN FROM DISCOSOMA SP. RED

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	1999-04-21
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97887,0.97868,0.95373
Spacegroup name	P 1 21 1
Unit cell lengths	56.366, 112.270, 70.691
Unit cell angles	90.00, 93.81, 90.00

Refinement procedure

Resolution	25.100 * - 2.000
R-factor	0.162 *
Rwork	0.162
R-free	0.24900
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	1.859
Data reduction software	MOSFLM
Data scaling software	FHSCALE
Phasing software	SHARP
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.100 *	2.100
High resolution limit [Å]	2.000	2.000
Rmerge	0.027 *	0.044 *
Total number of observations	232348 *
Number of reflections	56118 *
<I/σ(I)>	18.6
Completeness [%]	94.8	95.1
Redundancy	4.1	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.9 *	298	used microseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	beta-mercaptoethanol	2 (mM)
2	1	drop		300 (mM)
3	1	drop	Tris	20 (mM)
4	1	drop	protein	15 (mg/ml)
5	1	reservoir	PEG1550	16 (%)
6	1	reservoir	Tris	0.1 (M)