1G7J

CRYSTAL STRUCTURE OF HEN EGG WHITE LYSOZYME (HEL) COMPLEXED WITH THE MUTANT ANTI-HEL MONOCLONAL ANTIBODY D1.3 (VLW92H)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	SIEMENS
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	123.020, 59.150, 56.170
Unit cell angles	90.00, 113.96, 90.00

Refinement procedure

Resolution	15.000 - 1.750
R-factor	0.1974 *
Rwork	0.197
R-free	0.22850
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.698
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		1.790
High resolution limit [Å]	1.750 *	1.750
Rmerge	0.041	0.299
Total number of observations	69273 *
Number of reflections	35748
Completeness [%]	95.7	91.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	used macroseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	15-18 (%(w/v))
2	1	reservoir	potassium phosphate	0.1 (M)