1G7B
1.3 A STRUCTURE OF T3R3 HUMAN INSULIN AT 100 K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-03-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.8000 |
| Spacegroup name | H 3 |
| Unit cell lengths | 80.050, 80.050, 71.459 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.800 - 1.300 |
| R-factor | 0.176 * |
| Rwork | 0.176 |
| R-free | 0.20400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1trz |
| RMSD bond length | 0.010 |
| RMSD bond angle | 20.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.800 | 1.330 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.060 | 0.393 |
| Total number of observations | 912730 * | |
| Number of reflections | 41901 | |
| <I/σ(I)> | 48.8 | 8.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.5 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | microgravity * | 6.3 | 298 | 5 mg/ml human insulin, 0.01 M HCl, 0.007 M zinc acetate, 0.05 M sodium citrate, 17% acetone, 1.0 M NaCl. pH 6.3, SLOW COOLING at 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | insulin | 5 (mg/ml) | |
| 2 | 1 | 1 | 0.01 (M) | ||
| 3 | 1 | 1 | zinc acetate | 0.007 (M) | |
| 4 | 1 | 1 | sodium citrate | 0.05 (M) | |
| 5 | 1 | 1 | acetone | 17 (%) | |
| 6 | 1 | 1 | 1.0 (M) |
