1G74

Toward changing specificity: adipocyte lipid binding protein mutant, oleic acid bound form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	113
Detector technology	IMAGE PLATE
Collection date	2000-07-13
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	118.920, 36.890, 27.870
Unit cell angles	90.00, 92.61, 90.00

Refinement procedure

Resolution	25.000 - 1.700
Rwork	0.208
R-free	0.23700 *
Structure solution method	isomorphous with apo EF-ALBP (PDB 1G7N)
Starting model (for MR)	EF-ALBP (PDB 1G7N)
RMSD bond length	0.008 *
RMSD bond angle	1.400 *
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000 *	1.770
High resolution limit [Å]	1.700 *	1.710
Rmerge	0.046	0.304
Total number of observations	42285 *
Number of reflections	13235 *
<I/σ(I)>	16.4
Completeness [%]	93.9	90.1
Redundancy	3.19 *	3.37

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2 *	291	sodium/potassium phosphate, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	9.0 (mg/ml)
2	1	drop	HEPES	12.5 (M)	pH7.2
3	1	reservoir	sodium potassium phosphate	1.5-3 (M)
4	1	reservoir	PEG4000	24-34 (%)
5	1	reservoir		0.1-0.2 (M)	pH7.4
6	1	reservoir	ammonium sulfate	0.1 (M)