1G6U
CRYSTAL STRUCTURE OF A DOMAIN SWAPPED DIMER
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12B |
| Synchrotron site | NSLS |
| Beamline | X12B |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 0.975 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 64.040, 64.040, 64.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.480 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.24100 |
| Structure solution method | MIR |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | DM |
| Refinement software | X-PLOR (3.820) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.570 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.082 | 0.289 |
| Total number of observations | 80427 * | |
| Number of reflections | 14554 | |
| Completeness [%] | 97.9 | 94.1 * |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6.5 | 298 | 100 mM MES, pH 6.5, 100 mM NaCl, 2.6 M ammonium sulfate, 1% dioxane, VAPOR DIFFUSION, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | ammonium sulfate | 2.6 (M) | |
| 2 | 1 | 1 | MES | 100 (mM) | |
| 3 | 1 | 1 | 100 (mM) | ||
| 4 | 1 | 1 | dioxane | 1 (%) |
