1G6L
1.9A CRYSTAL STRUCTURE OF TETHERED HIV-1 PROTEASE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 296 |
Detector technology | IMAGE PLATE |
Spacegroup name | P 61 |
Unit cell lengths | 63.150, 63.150, 83.610 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 8.000 - 1.900 |
R-factor | 0.195 * |
Rwork | 0.195 |
R-free | 0.28400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 * |
RMSD bond angle | 1.800 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 1.900 |
Rmerge | 0.055 |
Total number of observations | 43302 * |
Number of reflections | 14318 |
Completeness [%] | 95.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 4.5 * | ||
1 | Vapor diffusion, hanging drop * | 4.5 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | enzyme | 6 (mg/ml) | |
2 | 1 | drop | sodium acetate | 50 (mM) | |
3 | 1 | reservoir | sodium phosphate | 198 (mM) | |
4 | 1 | reservoir | sodium citrate | 99 (mM) | |
5 | 1 | reservoir | ammonium sulfate | 10 (%sat) |