1G6H
CRYSTAL STRUCTURE OF THE ADP CONFORMATION OF MJ1267, AN ATP-BINDING CASSETTE OF AN ABC TRANSPORTER
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 96 |
| Detector technology | CCD |
| Collection date | 1999-05-07 |
| Detector | SIEMENS |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 107.807, 60.463, 42.730 |
| Unit cell angles | 90.00, 92.16, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.600 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.25200 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.670 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.048 * | |
| Number of reflections | 33512 | |
| <I/σ(I)> | 45.4 | |
| Completeness [%] | 92.3 | 85.2 |
| Redundancy | 10.4 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 37 * | used microseeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Mg-ADP | 20 (mM) | |
| 10 | 1 | reservoir | ethylene glycol | 15 (%(v/v)) | |
| 11 | 1 | reservoir | glycerol | 10 (%) | |
| 12 | 1 | reservoir | dithiothreitol | 0.1 (mM) | |
| 13 | 1 | reservoir | MES | 100 (mM) | |
| 2 | 1 | drop | protein | 36 (mg/ml) | |
| 3 | 1 | drop | PEG3350 | 16 (%) | |
| 4 | 1 | drop | ethylene glycol | 30 (%(v/v)) | |
| 5 | 1 | drop | glycerol | 20 (%(v/v)) | |
| 6 | 1 | drop | 4 (mM) | ||
| 7 | 1 | drop | dithiothreitol | 0.2 (mM) | |
| 8 | 1 | drop | MES | 200 (mM) | |
| 9 | 1 | reservoir | PEG3350 | 15-20 (%(v/v)) |
