1G67
THIAMIN PHOSPHATE SYNTHASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-11-01 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 76.980, 76.980, 140.150 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.990 - 1.400 |
| R-factor | 0.212 * |
| Rwork | 0.216 |
| R-free | 0.23600 * |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1g4e |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.990 | |
| High resolution limit [Å] | 1.400 | |
| Rmerge | 0.051 | 0.229 * |
| Number of reflections | 65601 * | |
| <I/σ(I)> | 7.9 | |
| Completeness [%] | 78.7 * | 64.3 * |
| Redundancy | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7.5 | 4 * | Chiu, H.-J., (1999) Biochemistry, 38, 6460. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | |
| 3 | 1 | drop | dithiothreitol | 2 (mM) | |
| 4 | 1 | reservoir | Tris-HCl | 75 (mM) | |
| 5 | 1 | reservoir | 75 (mM) | ||
| 6 | 1 | reservoir | PEG4000 | 24-26 (%(v/v)) | |
| 7 | 1 | reservoir | beta-mercaptoethanol | 2 (mM) | |
| 8 | 1 | reservoir | beta-octylglucopyranoside | 5 (mM) |
