1G63
PEPTIDYL-CYSTEINE DECARBOXYLASE EPID
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 90 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-01-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.09 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 164.680, 110.030, 152.870 |
| Unit cell angles | 90.00, 90.35, 90.00 |
Refinement procedure
| Resolution | 14.990 - 2.500 |
| R-factor | 0.23 |
| Rwork | 0.230 |
| R-free | 0.26300 |
| Structure solution method | MIR |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | CCP4 |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.560 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.058 | 0.248 |
| Total number of observations | 213488 * | |
| Number of reflections | 81936 * | |
| <I/σ(I)> | 10.2 | 2.8 |
| Completeness [%] | 87.1 | 73.3 |
| Redundancy | 2.6 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8 * | 18 * | 100 mM MES/NaOH 30% MPD , pH 6.4, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | MPD | 12 (%) | |
| 2 | 1 | reservoir | 10 (mM) | ||
| 3 | 1 | reservoir | Tris-HCl | 0.1 (M) |
