1G60
Crystal Structure of Methyltransferase MboIIa (Moraxella bovis)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-08-29 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 1.00599 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.818, 98.699, 50.894 |
| Unit cell angles | 90.00, 101.93, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 1.740 |
| R-factor | 0.198 * |
| Rwork | 0.198 |
| R-free | 0.22100 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.027 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 * | 1.770 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.066 | 0.339 |
| Number of reflections | 60068 * | |
| <I/σ(I)> | 12.1 | 3.4 |
| Completeness [%] | 99.7 | 99.4 |
| Redundancy | 3.72 | 3.63 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 277 | 4% PEG monomethyl ether 5000, 50 mM potassium chloride, 50 mM sodium chloride, 1 mM DTT, 50 mM bicine, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.5 (mM) | |
| 2 | 1 | drop | Tris-HCl | 5 (mM) | pH7.5 |
| 3 | 1 | drop | 75 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | reservoir | Bicine | 100 (mM) | pH8.4 |
| 6 | 1 | reservoir | PEG5000 MME | 4 (%) | |
| 7 | 1 | reservoir | 50 (mM) | ||
| 8 | 1 | reservoir | 50 (mM) | ||
| 9 | 1 | reservoir | dithiothreitol | 1 (mM) |
