1G5X
The Structure of Beta-Ketoacyl-[Acyl Carrier Protein] Synthase I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR571 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-12-13 |
Detector | MACSCIENCE |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.100, 139.600, 212.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.900 - 2.450 |
R-factor | 0.199 * |
Rwork | 0.199 |
R-free | 0.26600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | beta-ketoacyl acyl carrier protein synthase II |
RMSD bond length | 0.008 |
RMSD bond angle | 1.350 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.900 | 2.490 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.070 | 0.247 |
Number of reflections | 64781 | |
<I/σ(I)> | 21.5 | 5.7 |
Completeness [%] | 96.4 | 95.3 |
Redundancy | 3.1 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | ammonium sulfate, PEG 400, tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
2 | 1 | reservoir | PEG400 | 20 (%) | |
3 | 1 | reservoir | Tris | 100 (mM) |