1G5N
ANNEXIN V COMPLEX WITH HEPARIN OLIGOSACCHARIDES
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 1999-09-25 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.979 |
| Spacegroup name | H 3 |
| Unit cell lengths | 155.874, 155.874, 37.344 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 100.000 - 1.900 |
| R-factor | 0.205 * |
| Rwork | 0.208 |
| R-free | 0.23600 * |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | Unpublished structure of wild-type annexin V |
| RMSD bond length | 0.005 * |
| RMSD bond angle | 1.090 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.020 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.086 | 0.219 |
| Total number of observations | 144885 * | |
| Number of reflections | 26655 * | |
| <I/σ(I)> | 16.5 | |
| Completeness [%] | 92.1 | 93.5 |
| Redundancy | 7 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8.2 | 17 * | Campos, B., (1998) Biochemistry, 37, 8004. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 14 (mg/ml) | |
| 2 | 1 | drop | calcium | 20 (mM) | |
| 3 | 1 | reservoir | ammonium sulfate | 34-40 (%sat) | |
| 4 | 1 | reservoir | HEPPS | 50 (mM) | |
| 5 | 1 | reservoir | 0.02 (%) | ||
| 6 | 1 | reservoir | dithiothreitol | 2 (mM) | |
| 7 | 1 | reservoir | 10 (mM) |
