1G59
GLUTAMYL-TRNA SYNTHETASE COMPLEXED WITH TRNA(GLU).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-02-20 |
| Detector | FUJI |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 109.980, 218.670, 134.670 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.400 |
| R-factor | 0.218 * |
| Rwork | 0.218 |
| R-free | 0.29800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gln |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.430 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.109 | 0.340 |
| Total number of observations | 251910 * | |
| Number of reflections | 56248 | |
| <I/σ(I)> | 6.5 | |
| Completeness [%] | 88.5 | 74 * |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 293 | PEG1500, ammonium sulfate, Mops-Na, MPD, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG1500 | ||
| 2 | 1 | 1 | ammonium sulfate | ||
| 3 | 1 | 1 | Mops-Na | ||
| 4 | 1 | 1 | MPD | ||
| 5 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 22 (%) | ||
| 2 | 1 | reservoir | MOPS-Na | 37 (mM) | |
| 3 | 1 | reservoir | ammonium sulfate | 37 (mM) | |
| 4 | 1 | reservoir | 1 (%) | ||
| 5 | 1 | reservoir | 10 (mM) | ||
| 6 | 1 | reservoir | 2-mercaptoethanol | 5 (mM) |
