1G51
ASPARTYL TRNA SYNTHETASE FROM THERMUS THERMOPHILUS AT 2.4 A RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-04-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.135, 155.500, 171.145 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.000 - 2.400 |
| R-factor | 0.194 |
| Rwork | 0.194 |
| R-free | 0.25100 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 24.300 * |
| Data reduction software | XDS |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 11.500 | 2.450 |
| High resolution limit [Å] | 2.600 | 2.400 |
| Rmerge | 0.053 | 0.285 |
| Number of reflections | 60454 | |
| <I/σ(I)> | 22.15 | |
| Completeness [%] | 95.4 | 87.6 |
| Redundancy | 3.11 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 271 | seeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | sodium formate | 2 (M) | |
| 3 | 1 | drop | Tris-HCl | 50 (mM) | |
| 4 | 1 | drop | Tris-HCl | 50 (mM) | |
| 5 | 1 | reservoir | sodium formate | 2 (M) |
