1G4X

ASPARTATE AMINOTRANSFERASE ACTIVE SITE MUTANT N194A/R292L

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1995-08-15
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	155.040, 88.750, 80.070
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.200
R-factor	0.229
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	2.748
Phasing software	X-PLOR
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.140
Rmerge	0.065 *
Total number of observations	144708 *
Number of reflections	34702
Completeness [%]	90.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	Sodium Sulfate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	40-60 (mg/ml)
2	1	drop	potassium phosphate	10 (mM)
3	1	drop	PLP	0.010 (mM)
4	1	drop		0.3 (mM)
5	1	reservoir	potassium phosphate	10 (mM)
6	1	reservoir	PLP	0.010 (mM)
7	1	reservoir		0.3 (mM)
8	1	reservoir	ammonium sulfate	40 (%sat)