1G4M
CRYSTAL STRUCTURE OF BOVINE BETA-ARRESTIN 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-04-30 |
| Detector | BRANDEIS - B4 |
| Wavelength(s) | 0.979, 1.10 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.400, 73.717, 115.760 |
| Unit cell angles | 90.00, 98.73, 90.00 |
Refinement procedure
| Resolution | 34.540 - 1.900 |
| R-factor | 0.235 |
| Rwork | 0.235 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT, SIRAS |
| Starting model (for MR) | 1cf1 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 * | |
| High resolution limit [Å] | 1.960 * | |
| Rmerge | 0.047 * | 0.468 * |
| Total number of observations | 158905 * | |
| Number of reflections | 49309 * | |
| Completeness [%] | 65.8 * | |
| Redundancy | 3.2 * | 1.5 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 8.5 * | 19-20 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 5 (mg/ml) | or 7mg/ml |
| 2 | 1 | 1 | 0.1 (M) | ||
| 3 | 1 | 1 | Tris-HCl | 20 (mM) | |
| 4 | 1 | 1 | EDTA | 1 (mM) | |
| 5 | 1 | 1 | dithiothreitol | 10 (mM) | |
| 6 | 1 | 1 | glycerol | 5 (%) | |
| 7 | 1 | 2 | PEG4000 | 12-14.5 (%) | |
| 8 | 1 | 2 | 0.4 (M) | ||
| 9 | 1 | 2 | Tris-HCl | 0.1 (M) |
