1G3V
CRYSTAL STRUCTURE OF NICKEL-D[CGTGTACACG]2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X31 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-11-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 53.031, 53.031, 97.787 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 3.100 |
| R-factor | 0.209 * |
| Rwork | 0.209 |
| R-free | 0.27100 * |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.180 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.100 | 0.490 |
| Total number of observations | 43286 * | |
| Number of reflections | 2843 | |
| <I/σ(I)> | 20.3 | |
| Completeness [%] | 79.3 * | 50.2 * |
| Redundancy | 15.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | DNA, NiCl2, MPD, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 20K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | DNA | ||
| 2 | 1 | 1 | NiCl2 | ||
| 3 | 1 | 1 | MPD | ||
| 4 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.2 (mM) | |
| 2 | 1 | drop | 10 (mM) | ||
| 3 | 1 | drop | 4 (%) | ||
| 4 | 1 | drop | sodium cacodylate | pH6. | |
| 5 | 1 | reservoir | MPD | 10 (%) |
