1G38
ADENINE-SPECIFIC METHYLTRANSFERASE M. TAQ I/DNA COMPLEX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-04-07 |
Detector | MARRESEARCH |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 59.500, 68.650, 114.430 |
Unit cell angles | 90.00, 92.20, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.000 |
R-factor | 0.196 * |
Rwork | 0.196 |
R-free | 0.23400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2adm |
RMSD bond length | 0.006 |
RMSD bond angle | 1.400 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.130 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.088 | 0.195 |
Total number of observations | 218047 * | |
Number of reflections | 54457 | |
<I/σ(I)> | 10.42 | 3.6 |
Completeness [%] | 87.1 | 67.1 |
Redundancy | 4 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.3 * | 20 * | 15% Isopropanol, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | isopropanol |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | M-Taql | 0.075 (mM) | |
2 | 1 | drop | oligodeoxynucleotide | 0.100 (mM) | |
3 | 1 | drop | AETA | 2 (mM) | |
4 | 1 | drop | Tris-HCl | 10 (mM) | |
5 | 1 | drop | 300 (mM) | ||
6 | 1 | reservoir | sodium cacodylate | 50 (mM) | |
7 | 1 | reservoir | 100 (mM) | ||
8 | 1 | reservoir | 25 (mM) | ||
9 | 1 | reservoir | iso-propanol | 15 (%(v/v)) |