1G31
GP31 CO-CHAPERONIN FROM BACTERIOPHAGE T4
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1997-01 |
| Detector | PRINCETON 2K |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 157.677, 157.677, 90.932 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.225 |
| Rwork | 0.225 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ESCHERICHIA COLI GROES 2.8 ANGSTROM MODEL |
| RMSD bond length | 0.012 |
| RMSD bond angle | 25.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.108 * | |
| Number of reflections | 42831 | |
| <I/σ(I)> | 10.6 | 1.9 |
| Completeness [%] | 83.5 | 65 |
| Redundancy | 5.95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7 * | 21 * | PROTEIN WAS CRYSTALLIZED AT 17 MG/ML FROM 0.42 M NAH(2)PO(4), 1.70 M K(2)HPO(4), 10 MM BES, 6 MM DTT, 0.035% NAN(3), 29% ETHYLENE GLYCOL. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 17 (mg/ml) | |
| 2 | 1 | drop | dithiothreitol | 4 (mM) | |
| 3 | 1 | drop | BES | 10 (mM) | |
| 4 | 1 | reservoir | dithiothreitol | 2 (mM) | |
| 5 | 1 | reservoir | sodium phospahte | 0.42 (M) | |
| 6 | 1 | reservoir | potassium phosphate | 1.70 (M) | |
| 7 | 1 | reservoir | ethylene glycol | 29 (%(v/v)) | |
| 8 | 1 | reservoir | 0.035 (%(w/v)) |
