1G2Z
DIMERIZATION DOMAIN OF HNF-1ALPHA WITH A LEU 13 SELENOMETHIONINE SUBSTITUTION
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 1999-10-28 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.95372, 0.97957, 0.9798, 1.00 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 37.100, 41.200, 42.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.500 - 1.150 |
R-factor | 0.223 * |
Rwork | 0.216 |
R-free | 0.25300 * |
Structure solution method | MAD |
Starting model (for MR) | wARP model |
RMSD bond length | 0.013 * |
RMSD bond angle | 1.740 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | SHARP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.500 | 1.190 |
High resolution limit [Å] | 1.150 | 1.050 * |
Rmerge | 0.066 * | 0.398 * |
Number of reflections | 31540 * | |
<I/σ(I)> | 16.2 | 5.7 |
Completeness [%] | 93.0 * | 82.1 * |
Redundancy | 4.3 * | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 8.5 | 277 | PEG 4000, Tris-HCl, lithium sulphate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | peptide | 10 (mg/ml) | |
2 | 1 | reservoir | PEG4000 | 30 (%) | |
3 | 1 | reservoir | Tris-HCl | 100 (mM) | |
4 | 1 | reservoir | 0.2 (M) |