1G2Y
HNF-1ALPHA DIMERIZATION DOMAIN, WITH SELENOMETHIONINE SUBSTITUED AT LEU 12
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 1999-10-28 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.95372, 0.97957, 0.9798, 1.07812 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.270, 47.880, 40.470 |
| Unit cell angles | 90.00, 93.60, 90.00 |
Refinement procedure
| Resolution | 30.880 - 1.000 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| R-free | 0.19800 |
| Structure solution method | MAD |
| Starting model (for MR) | wARP model |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.900 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.900 | 1.050 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.049 | 0.101 |
| Number of reflections | 55221 | |
| <I/σ(I)> | 16.8 | 9.2 |
| Completeness [%] | 86.4 | 73.1 |
| Redundancy | 2.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8.5 | 277 | PEG 4000, Tris-HCl, lithium sulphate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | peptide | 10 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 30 (%) | |
| 3 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 4 | 1 | reservoir | 0.2 (M) |
