1G2U

THE STRUCTURE OF THE MUTANT, A172V, OF 3-ISOPROPYLMALATE DEHYDROGENASE FROM THERMUS THERMOPHILUS HB8 : ITS THERMOSTABILITY AND STRUCTURE.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1998-10-12
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.5418
Spacegroup name	P 32 2 1
Unit cell lengths	78.680, 78.680, 157.690
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 2.200 *
R-factor	0.185
Rwork	0.185
R-free	0.23000
RMSD bond length	0.006
RMSD bond angle	1.341 *
Phasing software	X-PLOR
Refinement software	CNS (1.0)

Data quality characteristics

	Overall
High resolution limit [Å]	2.100 *
Rmerge	89.400 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	Sakurai, M., (1992) J.Biochem., 112, 173. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	potassium phosphate	20 (mM)	pH7.6
3	1	reservoir	ammonium sulfate	1.0-2.0 (M)