1G2Q
CRYSTAL STRUCTURE OF ADENINE PHOSPHORIBOSYLTRANSFERASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-02-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.661, 45.767, 53.883 |
| Unit cell angles | 95.69, 108.40, 109.58 |
Refinement procedure
| Resolution | 20.000 - 1.500 |
| R-factor | 0.199 * |
| Rwork | 0.199 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g2p |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.345 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.024 * | 0.176 * |
| Total number of observations | 132505 * | |
| Number of reflections | 54848 | |
| <I/σ(I)> | 34.4 | 4.3 |
| Completeness [%] | 95.8 | 89.9 |
| Redundancy | 2.4 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5.5 | 298 | PEG 5000 MME, sodium acetate, citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium citrate | 100 (mM) | pH5.5 |
| 2 | 1 | reservoir | sodium acetate | 0.2 (M) | |
| 3 | 1 | reservoir | PEG5000 MME | 20 (%) |
