1G2Q
CRYSTAL STRUCTURE OF ADENINE PHOSPHORIBOSYLTRANSFERASE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9B |
Synchrotron site | NSLS |
Beamline | X9B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-02-14 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 |
Unit cell lengths | 42.661, 45.767, 53.883 |
Unit cell angles | 95.69, 108.40, 109.58 |
Refinement procedure
Resolution | 20.000 - 1.500 |
R-factor | 0.199 * |
Rwork | 0.199 |
R-free | 0.22500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1g2p |
RMSD bond length | 0.006 |
RMSD bond angle | 1.345 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.024 * | 0.176 * |
Total number of observations | 132505 * | |
Number of reflections | 54848 | |
<I/σ(I)> | 34.4 | 4.3 |
Completeness [%] | 95.8 | 89.9 |
Redundancy | 2.4 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 5.5 | 298 | PEG 5000 MME, sodium acetate, citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | sodium citrate | 100 (mM) | pH5.5 |
2 | 1 | reservoir | sodium acetate | 0.2 (M) | |
3 | 1 | reservoir | PEG5000 MME | 20 (%) |