1G2P
CRYSTAL STRUCTURE OF ADENINE PHOSPHORIBOSYLTRANSFERASE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9B |
Synchrotron site | NSLS |
Beamline | X9B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-07-11 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.98 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 49.426, 96.670, 112.075 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.750 |
R-factor | 0.208 * |
Rwork | 0.208 |
R-free | 0.23000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qb8 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.433 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.780 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.041 * | 0.240 * |
Total number of observations | 103449 * | |
Number of reflections | 26348 | |
<I/σ(I)> | 29.6 | 3.8 |
Completeness [%] | 95.5 | 82.8 |
Redundancy | 3.9 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.5 | 18 * | lithium sulfate, Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 13-15 (mg/ml) | |
2 | 1 | reservoir | HEPES | 100 (mM) | pH7.5 |
3 | 1 | reservoir | lithium sulfate | 1.5 (M) |