1G2J
RNA OCTAMER R(CCCP*GGGG) CONTAINING PHENYL RIBONUCLEOTIDE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-03-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 24.310, 24.310, 123.790 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.970 |
| Rwork | 0.217 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | RNA tetramer duplex |
| RMSD bond length | 0.009 |
| RMSD bond angle | 29.470 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.040 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.046 | 0.243 |
| Total number of observations | 25862 * | |
| Number of reflections | 1904 | |
| <I/σ(I)> | 20.5 | 17.3 |
| Completeness [%] | 99.1 | 99.5 |
| Redundancy | 13.6 | 11.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | Oligonucleotide, Calcium Acetate, Sodium Cacodylate, PEG 8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Oligonucleotide | ||
| 2 | 1 | 1 | Calcium Acetate | ||
| 3 | 1 | 1 | Sodium Cacodylate | ||
| 4 | 1 | 1 | PEG 8000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | RNA | 1.5 (mM) | |
| 2 | 1 | drop | calcium acetate | 50 (mM) | |
| 3 | 1 | drop | sodium cacodylate | 25 (mM) | |
| 4 | 1 | drop | PEG8000 | 4.5 (%(v/v)) | |
| 5 | 1 | reservoir | calcium acetate | 0.2 (M) | |
| 6 | 1 | reservoir | sodium cacodylate | 0.1 (M) | |
| 7 | 1 | reservoir | PEG8000 | 18 (%) |
