1G2F

STRUCTURE OF A CYS2HIS2 ZINC FINGER/TATA BOX COMPLEX (TATAZF;CLONE #6)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	140
Detector technology	CCD
Collection date	2000-03-15
Detector	ADSC QUANTUM 4
Wavelength(s)	1.5418
Spacegroup name	P 64
Unit cell lengths	104.800, 104.800, 104.200
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.000
R-factor	0.233 *
Rwork	0.233
R-free	0.26300
Structure solution method	MIR
Starting model (for MR)	Zif268
RMSD bond length	0.006
RMSD bond angle	1.100
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.074 *	0.567 *
Total number of observations	496779 *
Number of reflections	43331
<I/σ(I)>	28.52	6.58
Completeness [%]	99.5	96.2
Redundancy	11.46	2.14

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	PEG 400; NaCl; Bis-Tris Propane; CoCl2, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	PEG 400
2	1	1	NaCl
3	1	1	Bis-Tris Propane
4	1	1	CoCl2

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	1 (mM)
2	1	drop	DNA	1.1 (mM)
3	1	drop	Bis-Tris-propane	100 (mM)
4	1	reservoir	PEG400	30-35 (%)
5	1	reservoir		100-200 (mM)
6	1	reservoir	Bis-Tris propane	10-50 (mM)