1G2E
CRYSTAL STRUCTURE OF HUD AND AU-RICH ELEMENT OF THE TUMOR NECROSIS FACTOR ALPHA RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-04-05 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.370, 100.150, 33.270 |
| Unit cell angles | 90.00, 106.59, 90.00 |
Refinement procedure
| Resolution | 30.380 - 2.300 |
| R-factor | 0.174 |
| Rwork | 0.174 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fxl |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.280 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.380 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.106 * | |
| Number of reflections | 8327 | |
| <I/σ(I)> | 8.6 | 2.1 |
| Completeness [%] | 97.7 | 83.8 |
| Redundancy | 3 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 20 * | PEG 3350, Ammonium sulfate, Calcium chloride, Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 3350 | ||
| 2 | 1 | 1 | (NH4)2SO4 | ||
| 3 | 1 | 1 | CaCl2 | ||
| 4 | 1 | 1 | Tris |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG3350 | 30 (%(w/v)) | |
| 2 | 1 | reservoir | Tris | 50 (mM) | |
| 3 | 1 | reservoir | 200 (mM) | ||
| 4 | 1 | reservoir | 10 (mM) |
