1G2C
HUMAN RESPIRATORY SYNCYTIAL VIRUS FUSION PROTEIN CORE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-07-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9816 |
| Spacegroup name | P 1 |
| Unit cell lengths | 67.904, 71.539, 76.455 |
| Unit cell angles | 81.34, 73.80, 60.72 |
Refinement procedure
| Resolution | 10.000 - 2.300 |
| R-factor | 0.233 |
| Rwork | 0.233 |
| R-free | 0.28600 |
| Starting model (for MR) | modified polyser SV5 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.036 | 0.240 |
| Total number of observations | 119905 * | |
| Number of reflections | 56973 | |
| <I/σ(I)> | 8.9 | |
| Completeness [%] | 91.2 | 64.7 |
| Redundancy | 1.3 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | PEG 4000, Tris-HCl, Li2SO4, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 24-26 (%) | |
| 3 | 1 | reservoir | Tris-HCl | 200 (mM) | |
| 4 | 1 | reservoir | 300 (mM) |
