1G2B
ALPHA-SPECTRIN SRC HOMOLOGY 3 DOMAIN, CIRCULAR PERMUTANT, CUT AT N47-D48
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 31.100, 42.970, 53.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.000 - 1.120 |
| R-factor | 0.136 * |
| R-free | 0.20200 |
| Structure solution method | AB INITIO |
| RMSD bond length | 0.026 |
| RMSD bond angle | 0.039 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.000 | 1.200 |
| High resolution limit [Å] | 1.120 | 1.120 |
| Rmerge | 0.071 | 0.140 |
| Total number of observations | 102998 * | |
| Number of reflections | 24461 | |
| Completeness [%] | 93.4 | 91.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | Viguera, A.R., (1996) Nature Struct. Biol., 3, 874. * |
