1G28
STRUCTURE OF A FLAVIN-BINDING DOMAIN, LOV2, FROM THE CHIMERIC PHYTOCHROME/PHOTOTROPIN PHOTORECEPTOR PHY3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 2000-04-24 |
Detector | RIGAKU RAXIS IIC |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 |
Unit cell lengths | 44.890, 54.078, 70.619 |
Unit cell angles | 93.32, 94.01, 90.06 |
Refinement procedure
Resolution | 35.160 - 2.730 |
R-factor | 0.247 |
Rwork | 0.247 |
R-free | 0.27200 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 24.200 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.160 | 2.900 |
High resolution limit [Å] | 2.730 | 2.730 |
Rmerge | 0.115 | 0.443 |
Number of reflections | 16249 | |
<I/σ(I)> | 14.3 | |
Completeness [%] | 92.5 | 86.1 |
Redundancy | 1.8 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 8 | 20 * | PEG 5000 MME, Tris, glycerol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | Tris-HCl | 50 (mM) | |
2 | 1 | reservoir | mPEG5000 | 24 (%(w/v)) | |
3 | 1 | reservoir | glycerol | 12 (%(v/v)) |