1G23
THE STRUCTURAL BASIS OF THE CATALYTIC MECHANISM AND REGULATION OF GLUCOSE-1-PHOSPHATE THYMIDYLYLTRANSFERASE (RMLA). GLUCOSE-1-PHOSPHATE COMPLEX.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-03-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.885 |
| Spacegroup name | P 1 |
| Unit cell lengths | 71.583, 73.901, 133.748 |
| Unit cell angles | 89.81, 80.31, 80.18 |
Refinement procedure
| Resolution | 72.550 - 2.800 |
| R-factor | 0.2011 |
| Rwork | 0.199 |
| R-free | 0.22600 |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.270 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SOLVE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.870 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.024 | 0.089 |
| Total number of observations | 224654 * | |
| Number of reflections | 121112 | |
| <I/σ(I)> | 29.7 | 9.8 |
| Completeness [%] | 92.7 | 69.4 |
| Redundancy | 1.9 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 * | Blankenfeldt, W., (2000) Acta Crystallogr.,Sect.D, 56, 1501. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG6000 | 9-12 (%(w/v)) | |
| 2 | 1 | reservoir | lithium sulfate | 0.5 (M) | |
| 3 | 1 | reservoir | citrate/NaOH | 0.1 (M) |
