1G1V
T4 LYSOZYME MUTANT C54T/C97A/I58T
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1998-04-08 |
| Detector | UCSD MARK III |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 60.933, 60.933, 96.803 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| Starting model (for MR) | 1l63 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 15.520 * |
| Data reduction software | SDMS |
| Data scaling software | SDMS |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.050 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.058 | 0.159 |
| Number of reflections | 16299 | |
| <I/σ(I)> | 13.3 | 3 |
| Completeness [%] | 96.3 | 90.2 |
| Redundancy | 3.19 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.1 | 277 | Eriksson, A.E., (1993) J. Mol. Biol., 229, 747. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | 0.25 (M) | ||
| 3 | 1 | reservoir | phosphate | 2.0 (M) | |
| 4 | 1 | reservoir | beta-mercaptoethanol |
