1G1K
COHESIN MODULE FROM THE CELLULOSOME OF CLOSTRIDIUM CELLULOLYTICUM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID2 |
Synchrotron site | ESRF |
Beamline | ID2 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-02-22 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.500, 57.250, 105.720 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.000 |
R-factor | 0.23 |
Rwork | 0.230 |
R-free | 0.27000 |
RMSD bond length | 0.014 |
RMSD bond angle | 2.000 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.060 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.082 | 0.275 |
Number of reflections | 22829 | |
<I/σ(I)> | 6.4 | |
Completeness [%] | 98.0 | 98.8 * |
Redundancy | 3.5 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 20 * | used macroseeding * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | reservoir | PEG8000 | 30 (%(w/v)) | |
3 | 1 | reservoir | ammonium sulfate | 150 (mM) |