1G18
RECA-ADP-ALF4 COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-07-11 |
| Detector | MARRESEARCH |
| Spacegroup name | P 61 |
| Unit cell lengths | 107.988, 107.988, 72.006 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 3.800 |
| R-factor | 0.22 * |
| Rwork | 0.215 |
| R-free | 0.27700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.940 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmerge | 0.145 | 0.519 |
| Number of reflections | 4738 | |
| <I/σ(I)> | 14.5 | 3.5 |
| Completeness [%] | 98.0 | 99.6 |
| Redundancy | 4 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Phosphate-citrate buffer, PEG 4000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | phosphate-citrate | 100 (mM) | |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | drop | PEG4000 | 6 (%) | |
| 5 | 1 | reservoir | PEG4000 | 20 (%) | |
| 6 | 1 | reservoir | phosphate-citrate | 100 (mM) |
