1G15

CO-CRYSTAL OF E. COLI RNASE HI WITH TWO MN2+ IONS BOUND IN THE THE ACTIVE SITE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2000-07-02
Detector	MARRESEARCH
Spacegroup name	P 63
Unit cell lengths	71.653, 71.653, 48.127
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 1.900
Rwork	0.223
R-free	0.26100
RMSD bond length	0.006 *
RMSD bond angle	1.010 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.050
High resolution limit [Å]	1.900	1.900
Rmerge	0.083	0.420
Number of reflections	11149
<I/σ(I)>	4.2
Completeness [%]	99.8	99.3
Redundancy	4.95	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	294	1 mM MnCl2, 16 % PEG-3350, 20 mM Hepes, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6 (mg/ml)
2	1	reservoir		1 (mM)
3	1	reservoir	HEPES	20 (mM)
4	1	reservoir	PEG3350	16 (%)